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DFG-Forschergruppe FOR 721/2 Molekulare Struktur und Funktion der Tight Junction |
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Teilprojekt 5 |
Priv.-Doz. Dr. Ingolf Blasig
Leibniz-Institut für Molekulare Pharmakologie (FMP), Berlin-Buch
Alterationen von Occludin bei oxidativem Stress und Aufklärung der Funktion von Claudin-12
Es wird geprüft, ob Occludin die Tight Junction redoxabhängig beeinflusst. Nach CysteinAustausch soll unter Hypoxie untersucht werden, ob seine Oligomerisierung und Tight Junction-Funktion verändert ist.
Damit soll die Rolle von Cysteinen in Occludin hinsichtlich seiner Struktur und Funktion unter oxidativem Stress geklärt werden. Zu Claudin-12 werden Basiseigenschaften charakterisiert: Selbstassoziation, trans- und cis-Interaktion, Strangbildung sowie Barrierefunktion.
DFG-Mitarbeiter des Teilprojekts
Dipl.-Biol. Christian Bellmann (Doktorand)
Publikationen 2010 - 2012 (FOR 721/2)
Bellmann C, Schreivogel S, Günther R, Dabrowski S, Schümann M, Wolburg H, Blasig IE (2013) Highly conserved cysteines are involved in the oligomerization of occludin-redox dependency of the second extracellular loop. Antioxid. Redox Signal. 20(6): 855-867 (IF 7.7) [PubMed] [WebPage] [PDF] TP5
Cording J, Berg J, Käding N, Bellmann C, Tscheik C, Westphal JK, Milatz S, Günzel D, Wolburg H, Piontek J, Huber O, Blasig IE (201
Doerfel MJ, Westphal JK, Krug SM, Bellmann C, Blasig IE, Fromm M, Tauber R, Huber O (2013) CK2-dependent phosphorylation of occludin regulates the interaction with ZO-proteins and tight junction integrity. Cell Commun. Signal. 11(1): 40 (IF 4.7) [PubMed] [WebPage] [PDF] TP3+TP1+TP5+TPZ{2PI}
Hülper P, Veszelka S, Walter FR, Wolburg H, Fallie-Becker P, Piontek J, Blasig IE, Lakomek M, Kugler W, Deli MA (2013) Acute effects of short-chain alkylglycerols on blood-brain barrier properties of cultured brain endothelial cells. Brit. J. Pharmacol. ###: ###-### (IF 4.4)
Review
Tscheik C, Blasig IE, Winkler L (2013) Trends in drug delivery through tissue barriers containing tight junctions. Tissue Barriers 1(2): ###-### (new journal, no IF yet) [WebPage] [Full text WebPage] [pending: PDF] TP5
Bal MS*, Castro V* (*shared first autorship), Piontek J, Rueckert C, Walter JK, Shymanets A, Kurig B, Haase H, Nürnberg B, Blasig IE (2012) The hinge region of the scaffolding protein of cell contacts, zonula occludens protein regulates interacting with various signaling proteins. J. Cell. Biochem. 113(3): 934-945 (IF 3.1) [PubMed] [WebPage] [PDF]
Del Vecchio G*, Tscheik C* (*shared first authorship), Tenz K, Helms HC, Winkler L, Blasig R, Blasig IE (2012) Sodium caprate transiently opens claudin-5-containing barriers at tight junctions of epithelial and endothelial cells. Mol Pharm. 9(9): 2523-2533 (IF 4.8) [PubMed] [WebPage] [PDF]
Hackel D, Krug SM, Sauer RS, Mousa SA, Böcker A, Pflücke D, Wrede EJ, Kistner K, Hoffmann T, Niedermirtl B, Sommer C, Bloch L, Huber O, Blasig IE, Amasheh S, Reeh PW, Fromm M, Brack A, Rittner HL (2012) Transient opening of the perineurial barrier for analgesic drug delivery. Proc. Natl. Acad. Sci. USA 109(29): E2018-E2027 (IF 9.7) [PubMed] [WebPage] [PDF]
Kaufmann R, Piontek J° (°corresponding author), Grüll F, Kirchgessner M, Rossa J, Blasig IE, Cremer C (2012) Visualization and quantitative analysis of tight junction networks using localization microscopy. Plos One 7(2): e31128 (print pages 1-9) (IF 4.4) [PubMed] [WebPage] [PDF]
Rueckert C*, Castro V* (*shared first authorship), Gagell C, Dabrowski S, Schümann M, Krause E, Haseloff RF, Blasig IE (2012) Association between segments of zonula occludens proteins: Livecell FRET and mass spectrometric analysis. Ann. N.Y. Acad. Sci. 1257: 67-76 (IF 3.2) [PubMed] [WebPage] [PDF] (Original paper)
Walther W, Petkov S, Kuvardina ON, Aumann J, Kobelt D, Fichtner I, Lemm M, Piontek J, Blasig IE, Stein U, Schlag PM (2012) Novel Clostridium perfringens enterotoxin suicide gene therapy for selective treatment of claudin-3- and -4-overexpressing tumors Gene Ther. 19(5): 494-503 (IF 3.7) [PubMed] [WebPage] [PDF]
Zwanziger D, Hackel D, Staat C, Böcker A, Brack A, Beyermann M, Rittner HL, Blasig IE (2012) A peptidomimetic tight junction modulator to improve regional analgesia. Mol. Pharmaceut. 9(6): 1785-1794 (IF 5.4) [PubMed] [WebPage] [PDF]
Zwanziger D*, Staat C* (*shared first authorship), Andjelkovic AV, Blasig IE (2012) Claudin-derived peptides are internalized via specific
endocytosis pathways.
Ann. N.Y. Acad. Sci. 1257: 29-37
(IF 3.2) [PubMed] [WebPage]
[PDF] (Original paper) Reviews
Zhang J, Liss M, Wolburg H, Blasig IE, Abdelilah-Seyfried S (2012) Involvement of caudins in zebrafish brain ventricle morphogenesis. Ann. N.Y. Acad. Sci. 1257: 193-198 (IF 3.2) [PubMed] [WebPage] [PDF] (Review)
Piontek J, Fritzsche S, Cording J, Richter S, Hartwig J, Walter M, Yu D, Turner JR, Gehring C, Rahn HP, Wolburg H, Blasig IE (2011) Elucidating the principles of the molecular organization of heteropolymeric tight junction strands. Cell. Mol. Life Sci. 68(23): 3903-3918 [PubMed] [WebPage] [PDF]
Reviews
Blasig IE, Bellmann C, Cording J, del Vecchio G, Zwanziger D, Huber O, Haseloff RF (2011) Occludin protein family: oxidative stress and reducing conditions. Antioxid. Redox Signal. 15(5): 1195-1219 (IF 7.6) [PubMed] [WebPage] [PDF for printing] [PDF with links] (Review)
Blasig IE, Haseloff RF (2011) Tight junctions and tissue barriers. Antioxid. Redox Signal. 15(5): 1163-1166 (IF 8.2) [PubMed] [WebPage] [PDF for printing] [PDF with links] (Editorial)
Piehl C,* Piontek J* (*shared first authorship), Cording J, Wolburg H, Blasig IE (2010) Participation of the second extracellular loop of claudin-5 in paracellular tightening against ions, small and large molecules. Cell. Mol. Life Sci. 67(12): 2131-2140 [PubMed] [WebPage] [PDF]
Westphal JK, Dörfel MJ, Krug SM, Cording JD, Piontek J, Blasig IE, Tauber R, Fromm M, Huber O (2010) Tricellulin forms homomeric and heteromeric tight junctional complexes. Cell. Mol. Life Sci. 67(12): 2057-2068 [PubMed] [WebPage] [Supplement] [PDF]
Zhang J, Piontek J, Wolburg H, Piehl C, Liss M, Otten C, Christ A, Willnow TE, Blasig IE, Abdelilah-Seyfried S (2010) Establishment of a neuroepithelial barrier by claudin-5a is essential for zebrafish brain ventricular lumen expansion. Proc. Natl. Acad. Sci. 107(4): 1425-1430 [PubMed] [WebPage] [PDF]
Reviews
Haseloff RF, Piontek J, Blasig IE (2010) The investigation of cis- and trans-interactions between claudins. Curr. Top. Membr. 65: 97-112 [Directory] [WebPage] [PDF] (review / book chapter)
Veshnyakova A, Protze J, Rossa J, Blasig IE, Krause G, Piontek J (2010) On the interaction of clostridium perfringens enterotoxin with claudins. Toxins 2(6): 1336-1356 [Abstract] [PDF] (Review)
Vorarbeiten
Walter JK, Castro V, Voss M, Gast K, Rueckert C, Piontek J, Blasig IE (2009) Redox-sensitivity of the dimerization of occludin. Cell. Mol. Life Sci. 66(22): 3655-3662 [PubMed] [WebPage] [PDF]
Lohrberg D, Krause E, Schümann M, Piontek J, Winkler L, Blasig IE, Haseloff RF (2009) A strategy for enrichment of claudins based on their affinity to Clostridium perfringens enterotoxin. BMC Mol. Biol. 10(1): 61. [PubMed] [WebPage] [PDF] [Supplement]
Winkler L, Gehring C, Wenzel A, Müller SL, Piehl C, Krause G, Blasig IE*, Piontek J* (*shared last authorship) (2009) Molecular determinants of the interaction between clostridium perfringens enterotoxin fragments and claudin-3. J. Biol. Chem. 284(28): 18863-18872 [PubMed] [WebPage] [PDF]
Piontek J, Winkler L, Wolburg H, Müller SL, Zuleger N, Piehl C, Wiesner B, Krause G, Blasig IE (2008) Formation of tight junction: determinants of homophilic interaction between classic claudins. FASEB J. 22(1): 146-158. [PubMed] [WebPage] [PDF]
Schreibelt G, van Horssen J, Haseloff F, Reijerkerk A, van der Pol SMA, Niewenhuizen O, Krause E, Blasig IE, Dijkstra CD, Ronken E, de Vries HE (2008) Protective effects of peroxiredoxin-1 at the injured blood-brain barrier. Free Radical. Biol. Med. 45: 256-264.
Krause G, Winkler L, Mueller SL, Haseloff RF, Piontek J, Blasig IE (2008) Structure and function of claudins. Biochim. Biophys. Acta 1778(3): 631-645. [PubMed] [WebPage] [PDF]
Schroeter ML, Abdul-Khaliq H, Krebs M, Dieffenbacher A, Blasig IE (2008) Serum markers support disease-specific glial pathology in major depression. J. Affect. Disord. 111: 271-280.
Haqqani AS, Kelly J, Baumann J, Haseloff RF, Blasig IE,Stanimirovic DB (2007) Protein markers of ischemic insult in brain endothelial cells identified using 2 D gel electrophoresis and ICAT-based quantitative proteomics: Comparison of in vitro and in vivo models. J. Proteome Res. 6: 226-239.
Schreibelt G, Kooij G, Reijerkerk A, van Doorn R, Gringhuis SI, van der Pol SMA, We-ksler BB, Romero IE, Couraud PQ, Piontek J, Blasig IE, Dijkstra CD, Ronken E, de Vries HE (2007) Reactive oxygen species alter brain endothelial tight junction dynamics via RhoA, PI3 kinase and PKB signaling. FASEB J. 21: 3666-3676.
Andreeva AY, Piontek J, Blasig IE, Utepbergenov DI (2006) Assembly of tight junction is regulated by the antagonism of conventional and novel protein kinase C isoforms. Int. J. Biochem. Cell Biol. 38: 222-233.
Blasig IE, Winkler L, Lassowski B, Mueller SL, Zuleger N, Krause E, Krause G, Gast K, Kolbe M, Piontek J (2006) On the self-association potential of transmembrane tight junction proteins. Cell. Mol. Life Sci. 63: 505-514.
Sury MD, Freese-Schaper MK, Mühlemann FT, Schulthess FT, Blasig IE, Täuber MG, Shaw SG Christen S (2006) N-acetylcysteine inhibits TNF-alpha-induced up-regulation of endothelin-1 but not of inducible nitric oxide synthase in rat brain capillary endothelial cells. Free Radical Biol. Med. 41: 1372-1383.
Haseloff RF, Krause E, Bigl M, Mikoteit K, Stanimirovic D, Blasig IE (2006) Differential protein expression in brain capillary endothelial cells induced by hypoxia and posthypoxic reoxygenation. Proteomics 6: 1803-1809.
Mueller SL, Portwich M, Schmidt A, Utepbergenov DI, Huber O, Blasig IE, Krause G (2005) The tight junction protein occludin and the adherens junction protein a-catenin share a common interaction mechanism with ZO-1. J. Biol. Chem. 280: 3747-3756.
Haseloff, RF, Blasig IE, Bauer HC, Bauer H (2005) In search of the astrocytic factor(s) modulating blood-brain barrier functions in brain capillary endothelial cells in vitro. Cell. Mol. Neurobiol. 25: 25-39.
Schmidt A, Utepbergenov DI, Mueller SL, Beyermann M, Schneider-Mergener J, Krause G, Blasig IE (2004) Occludin binds to the SH3-hinge-GuK unit of zonula occludens protein 1 - potential mechanism of tight junction regulation. Cell. Mol. Life Sci. 61: 1354-1365.
Ann. N.Y. Acad. Sci. 1165 [Contents and Full Papers]
Krause G, Winkler L, Piehl C, Blasig I, Piontek J, Müller SL (2009) Structure and function of extracellular claudin domains. Ann. N.Y. Acad. Sci. 1165: 34-43 [PubMed] [WebPage] [PDF]
Walter JK, Rückert C, Voss M, Müller SL, Piontek J, Blasig I (2009) The oligomerization of the coiled-coil domain of occludin is redox-sensitive. Ann. N.Y. Acad. Sci. 1165: 19-27 [PubMed] [WebPage] [PDF]