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Publications: Jörg Piontek
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2025
Martínez-Perafán F, Fromm A, van der Veen RE, Waldow A, Lehmann M, Krug SM, Günzel D, Rosenthal R, Fromm M, Piontek J (2025) Effect of claudin-1 or -3 expression on cation and water channel properties of claudin-2.
Mathew-Schmitt S, Oerter S, Reitenbach E, Gätzner S, Höchner A, Jahnke HG, Piontek J, Neuhaus W, Brachner A, Metzger M, Appelt-Menzel A (2025) Generation of advanced blood-brain barrier spheroids using human-induced pluripotent stem cell-derived brain capillary endothelial-like cells.
Pouyiourou I, Fromm A, Piontek J, Rosenthal R, Furuse M*, Günzel D* (*shared last authorship) (2025) Ion permeability profiles of renal paracellular channel-forming claudins. Acta Physiol. 241: e14264 (18 pages). doi: 10.1111/apha.14264 (°IF 5.6)
2024
Nagarajan SK, Piontek J (2024) Molecular dynamics simulations of claudin-10a and -10b ion channels: With similar architecture, different pore linings determine the opposite charge selectivity. Int. J. Mol. Sci. 25(6): 3161 (23 pages), doi: 10.3390/ijms25063161 (IF 4.9)
Nagarajan SK, Weber J, Roderer D, Piontek J (2024) C. perfringens enterotoxin-claudin pore complex: Models for structure, mechanism of pore assembly and cation permeability. Comp. Struct. Biotechnol. J. 27: 287-306. doi: 10.1016/j.csbj.2024.11.048 (IF 4.1)
Legere EA, Baumholtz AI, Lachance JB, Archer M, Piontek J, Ryan AK (2024) Claudin-3 in the non-neural ectoderm is essential for neural fold fusion in chicken embryos. Dev. Biol. 507: 20-33. doi: 10.1016/j.ydbio.2023.12.009 (IF 2.1)
van der Veen RE, Piontek J, Bieck M, Saiti A, Gonschior H, Lehmann M (2024) Claudin-4 polymerizes after a small extracellular claudin-3-like substitution. J. Biol. Chem. 300(10): 107693 (14 pages). doi: 10.1016/j.jbc.2024.107693 (IF 3.9)
2023
Waldow A*, Beier LS* (*shared first authorship), Arndt J, Schallenberg S, Vollbrecht C, Bischoff P, Farrera-Sal M, Loch FN, Bojarski C, Schumann M, Winkler L, Kamphues C, Ehlen L°, Piontek J° (°shared last authorship) (2023) cCPE fusion proteins as molecular probes to detect claudins and tight junction dysregulation in gastrointestinal cell lines, tissue explants and patient-derived organoids. Pharmaceutics 15(7): 1980 (23 pages). doi: 10.3390/pharmaceutics15071980 (IF 4.9)
Nagarajan SK, Klein S, Fadakar BS, Piontek J (2023) Claudin-10b cation channels in tight junction strands: Octameric-interlocked pore barrels constitute paracellular channels with low water permeability Comp. Struct. Biotechnol. J. 21: 1711-1727, doi: 10.1016/j.csbj.2023.02.009 (IF 4.4)
2022
Beier LS*, Waldow A* (*shared first authorship), Khomeijani Farahani S, Mannweiler R, Vidal-Y-Sy S, Brandner JM, Piontek J°, Günzel D° (°shared last authorship) (2022) Claudin targeting as an effective tool for directed barrier modulation of the viable epidermis. Ann. NY Acad. Sci. 1517(1): 251-265, doi: 10.1111/nyas.14879 (IF 5.2)
Hempel C*, Rosenthal R* (*shared first authorship), Fromm A, Krug SM, Fromm M, Günzel D, Piontek J (2022) Tight junction channels claudin-10b and claudin-15: Functional mapping of pore-lining residues. Ann. NY Acad. Sci. 1515(1): 129-142, doi: 10.1111/nyas.14794 (IF 5.2)
Gonschior H, Schmied C, Van der Veen R, Eichhorst J, Himmerkus N, Piontek J, Günzel D, Bleich M, Furuse M, Haucke V, Lehmann M (2022) Nanoscale segregation of
channel and barrier claudins enables paracellular ion flux. Nat. Commun. 13(1): 4985 (20 pages). doi: 10.1038/s41467-022-32533-4,
Supplement (IF 16.6) Sewerin S, Piontek J, Schönauer R, Grunewald S, Rauch A, Neuber S, Bergmann C, Günzel D*, Halbritter J*
(*shared last authorship) (2022) Defective claudin-10 causes a novel variation of HELIX syndrome
through compromised tight junction strand assembly. Genes & Dis. 9(5): 1301-1314.
doi: 10.1016/j.gendis.2021.06.006 (IF
6.8)
Zejc T, Piontek J, Schulzke JD, Fromm M, Ervens J*, Rosenthal R* (*shared
last authorship) (2022)
Clinical significance of claudin expression in oral squamous cell carcinoma.
Int. J. Mol. Sci.
Book chapter
Beier LS, Piontek J, Piontek A, Protze J, Kobelt D, Walther W (2022) Claudin-targeted suicide gene therapy for claudin-overexpressing tumor cells by using modified Clostridium perfringens enterotoxin (CPE). In: Walter W (ed), Gene therapy of cancer. Methods Mol. Biol. 2521: 173-188. doi: 10.1007/978-1-0716-2441-8_9
2021
Dias MC, Quesada AO, Soldati S, Boesch F, Gruber I, Hildbrand T, Soenmez D, Khire T, Witz G, McGrath JL, Piontek J, Kondoh M, Deutsch U, Zuber B, Engelhardt B (2021) Brain endothelial tricellular junctions as novel sites for T-cell diapedesis across the blood-brain barrier. J. Cell Sci. 134(8): jcs253880 (19 pages), doi: org/10.1242/jcs.253880, Supplement PDF (IF 5.2)
Pahle J, Kobelt D, Aumann J, Behrens D, Daberkow O, Mokritzkij M, Piontek J, Stein U, Walther W (2021) Effective oncoleaking treatment of pancreatic cancer by claudin-targeted suicide gene therapy with Clostridium perfringens enterotoxin (CPE). Cancers 13(17): 4393 (26 pages). doi: 10.3390/cancers13174393, Supplement, (IF 6.6)
2020
Hempel C, Protze J, Altun E, Riebe B, Piontek A, Fromm A, Lee IM, Saleh T, Günzel D, Krause G, Piontek J (2020) Assembly of tight junction strands: Claudin-10b and claudin-3 form homo-tetrameric building blocks that polymerize in a channel-independent manner. J. Mol. Biol. 432(7): 2405-2427 (IF 5.5) [PubMed] [WebPage] [PDF] [Supplementary Figures S1-S11] [Supplementary Table S1]
Piontek A, Eichner M, Zwanziger D, Beier LS, Protze J, Walter W, Theurer S, Schmid KW, Führer-Sakel D, Piontek J*, Krause G* (*shared last authorship) (2020) Targeting claudin-overexpressing thyroid and lung cancer by modified Clostridium perfringens enterotoxin. Mol. Oncol. 14(2) :261-276 (IF 6.6) [PubMed] [WebPage] [PDF]
Rosenthal R, Günzel D, Piontek J, Krug SM, Ayala-Torres C, Hempel C, Theune D, Fromm M (2020) Claudin-15 forms a water channel through the tight junction with distinct function compared to claudin-2. Acta Physiol. 228(1): e13334 (15 pages) doi: 10.1111/apha.13334 (IF 6.3)
Review
Piontek J, Krug SM, Protze J, Krause G, Fromm M (2020) Molecular architecture and assembly of the tight junction backbone. BBA - Biomembranes 1862(7): 183279 (15 pages) (IF 3.7) [Part of Special Volume "The vertebrate epithelial apical junctional complex", Hervé JC ed.] doi: 10.1016/j.bbamem.2020.183279
2019
Beier LS, Rossa J, Woodhouse S, Bergmann S, Kramer HB, Protze J, Eichner M, Piontek A, Vidal-y-Sy S, Brandner JM, Krause G, Zitzmann N, Piontek J (2019) Use of modified Clostridium perfringens enterotoxin fragments for claudin targeting in liver and skin cells. Int. J. Mol. Sci. 20(19): 4774 (20 pages) (IF 4.6) [PubMed] [WebPage] [PDF] [Supplement PDF]
2018
Neuhaus W, Piontek A, Protze J, Eichner M, Mahringer A, Subileau EA, Lee IM, Schulzke JD, Krause G, Piontek J (2018) Reversible opening of the blood-brain barrier by claudin-5-binding variants of Clostridium perfringens enterotoxin's claudin-binding domain. Biomaterials 161: 129-143 (IF 10.3) [PubMed] [WebPage] [PDF]
2017
Piontek A, Rossa J, Protze J, Wolburg H, Hempel C, Günzel D, Krause G, Piontek J (2017) Polar and charged extracellular residues conserved among sealing claudins contribute to tight junction strand formation. Ann. N.Y. Acad. Sci. 1397: 143-156 (IF 4.3) [PubMed] [WebPage] [PDF]
Piontek A*, Witte C* (*shared first authorship), Rose H, Eichner M, Protze J, Krause G (corresponding author), Piontek J*, Schröder L* (*shared last authorship) (2017) A cCPE-based xenon biosensor for magnetic resonance imaging of claudin-expressing cells. Ann. N.Y. Acad. Sci. 1397: 195-208 (IF 4.3) [PubMed] [WebPage] [PDF]
Klar J, Piontek J, Milatz S, Tariq M, Jameel M, Breiderhoff T, Schuster J, Fatima A, Asif M, Sher M, Mäbert K, Fromm A, Baig SM, Günzel D, Dahl N (2017) Altered paracellular cation permeability due to a rare CLDN10B variant causes anhidrosis and kidney damage. Plos Genet. 13(7): e1006897 (22 pages + 6 Supplements) (IF 5.5) [PubMed] [WebPage] [PDF] [Supplement]
Baumholtz AI, Simard A, Nikolopoulou E, Oosenbrug M, Collins MM, Piontek A, Krause G, Piontek J, Greene NDE, Ryan AK (2017) Claudins are essential for cell shape changes and convergent extension movements during neural tube closure. Dev. Biol. 428(1): 25-38 (IF 3.3) [PubMed] [WebPage] [PDF]
Milatz S, Piontek J, Hempel C, Meoli L, Grohe C, Fromm A, Lee IM, El-Athman R, Günzel D (2017) Tight junction strand formation by claudin-10 isoforms and claudin-10a/-10b chimeras. Ann. N.Y. Acad. Sci. 1405: 102-115 (IF 4.3) [PubMed] [WebPage] [PDF]
Appelt-Menzel A, Cubukova A, Günther K, Edenhofer F, Piontek J, Krause G, Stüber T, Walles H, Neuhaus W, Metzger M (2017) Establishment of a human blood-brain barrier co-culture model mimicking the neurovascular unit using induced pluri- and multipotent stem cells. Stem Cell Rep.
Reviews
Eichner M, Protze J, Piontek A, Krause G, Piontek J (2017) Targeting and alteration of tight junctions by bacteria and their virulence factors such as Clostridium perfringens enterotoxin. Pflügers Arch. 469(1): 77-90 (IF 2.8)
Fromm M, Piontek J, Rosenthal R, Günzel D, Krug SM (2017) Tight junctions of the proximal tubule and their channel proteins. Pflügers Arch. 469(7-8): 877-887 (IF 2.8) [PubMed] [WebPage] [PDF]
2016
Conrad MP*, Piontek J* (*shared first authorship), Günzel D, Fromm M, Krug SM (2016) Molecular basis of claudin-17 anion selectivity. Cell. Mol. Life Sci. 73(1): 185-200 (IF 5.9) [PubMed] [WebPage] [PDF] [Supplement]
Liao Z, Yang Z, Piontek A, Eichner M, Krause G, Chen B, Li L, Piontek J, Zhang J (2016) Specific binding of a mutated fragment of Clostridium perfringens enterotoxin to endothelial claudin-5 and its modulation of cerebral vascular permeability. Neuroscience 327: 53-63 (IF 3.3) [PubMed] [WebPage] [PDF]
2015
Protze J, Eichner M, Piontek A, Dinter S, Rossa J, Blecharz KG, Vajkoczy P, Piontek J*, Krause G* (*shared last authorship) (2015) Directed structural modification of Clostridium perfringens enterotoxin to enhance binding to claudin-5. Cell. Mol. Life Sci. 72(7): 1417-1432 (IF 5.7) [PubMed] [WebPage] [PDF]
Zhang J*, Ni C*, Yang Z* (*shared first authorship), Piontek A, Chen H, Wang S, Fan Y, Qin Z, Piontek J (2015) Specific binding of Clostridium perfringens enterotoxin fragment 1 to claudin-b and modulation of zebrafish epidermal barrier. Exp. Dermatol. 24(8): 605-610 (IF 2.7) [PubMed] [WebPage] [PDF]
Milatz S, Piontek J, Schulzke JD, Blasig IE, Fromm M, Günzel D (2015) Probing the cis-arrangement of prototype tight junction proteins claudin-1 and claudin-3. Biochem. J. 468(3): 449-458 (IF 3.6) [PubMed] [WebPage] [PDF]
Review
Krause G, Protze J, Piontek J (2015) Assembly and function of claudins: Structure-function relationships based on homology models and crystal structures. Semin. Cell Devel. Biol. 42: 3-12 (IF 5.2) [PubMed] [WebPage] [PDF]
2014
Rossa J, Plöger C, Vorreiter F, Saleh T, Protze J, Günzel D, Wolburg H, Krause G, Piontek J (2014) Claudin-3 and claudin-5 protein folding and assembly into the tight junction are controlled by non-conserved residues in transmembrane2 (TM3) and extracellular loop 2 (ECL2) segments. J. Biol. Chem. 289(11): 7641-7653 (IF 4.6) [PubMed] [WebPage] [PDF] [Supplements]
Rossa J*, Protze J* (*shared first authorship), Kern C, Piontek A, Günzel D, Krause G*, Piontek J* (*shared last authorship) (2014) Molecular and structural transmembrane determinants critical for embedding claudin-5 into tight junctions reveal distinct four helix bundle arrangement. Biochem. J. 464(1): 49-60 (IF 4.4) [PubMed] [WebPage] [PDF]
2013
Cording J, Berg J, Käding N, Bellmann C, Tscheik C, Westphal JK, Milatz S, Günzel D, Wolburg H, Piontek J, Huber O, Blasig IE (2013) In tight junctions, claudins regulate the interactions between occludin, tricellulin and marvelD3, which, inversely, modulate the claudin oligomerization. J. Cell Sci. 126(2): 554-564 (IF 5.3) [PubMed] [WebPage] [PDF] [Supplement]
Hülper P, Veszelka S, Walter FR, Wolburg H, Fallier-Becker P, Piontek J, Blasig IE, Lakomek M, Kugler W, Deli MA (2013) Acute effects of short-chain alkylglycerols on blood-brain barrier properties of cultured brain endothelial cells. Br. J. Pharmacol. 169(7): 1561-1573 (IF 5.1) [PubMed] [WebPage] [PDF]
2012
Rossa J, Lorenz D, Ringling M, Veshnyakova A, Piontek J (2012) Overexpression of claudin-5 but not claudin-3 induces formation of transinteraction-dependent multilamellar bodies. Ann. N.Y. Acad. Sci. 1257: 59-66 (IF 4.4) [PubMed] [WebPage] [PDF] (Original paper)
Veshnyakova A*, Piontek J*° (*shared first authorship, °corresponding author), Protze J, Waziri N, Heise I, Krause G (2012) Mechanism of Clostridium perfringens enterotoxin interaction with claudin-3/-4 suggests structural modifications of the toxin to target specific claudin. J. Biol. Chem. 287(3): 1698-1708 (IF 4.7) [PubMed] [WebPage] [Full text + supplements PDF]
Veshnyakova A, Krug SM, Mueller SL, Piontek J, Protze J, Fromm M, Krause G (2012) Determinants contributing to claudin ion channel formation. Ann. N.Y. Acad. Sci. 1257: 45-53 (IF 4.4) [PubMed] [WebPage] [PDF] (Original paper)
Bal MS*, Castro V* (*shared first autorship), Piontek J, Rueckert C, Walter JK, Shymanets A, Kurig B, Haase H, Nürnberg B, Blasig IE (2012) The hinge region of the scaffolding protein of cell contacts, zonula occludens protein regulates interacting with various signaling proteins. J. Cell. Biochem. 113(3): 934-945 (IF 3.1) [PubMed] [WebPage] [PDF]
Kaufmann R, Piontek J, Grüll F, Kirchgessner M, Rossa J, Wolburg H, Blasig IE, Cremer C (2012) Visualization and quantitative analysis of reconstituted tight junctions using localization microscopy. PLOS One 7(2): e31128 (9 pages) (IF 3.7) [PubMed] [WebPage] [PDF]
Walther W, Petkov S, Kuvardina ON, Aumann J, Kobelt D, Fichtner I, Lemm M, Piontek J, Blasig IE, Stein U, Schlag PM (2012) Novel Clostridium perfringens enterotoxin suicide gene therapy for selective treatment of claudin-3- and -4-overexpressing tumors Gene Ther. 19(5): 494-503 (IF 4.3) [PubMed] [WebPage] [PDF]
2010 - 2011
Piontek J, Fritzsche S, Cording J, Richter S, Hartwig J, Walter M, Yu D, Turner JR, Gehring C, Rahn HP, Wolburg H, Blasig IE (2011) Elucidating the principles of the molecular organization of heteropolymeric tight junction strands. Cell. Mol. Life Sci. 68(23): 3903-3918 (IF 6.6) [PubMed] [WebPage] [PDF]
Piehl C,* Piontek J* (*shared first authorship), Cording J, Wolburg H, Blasig IE (2010) Participation of the second extracellular loop of claudin-5 in paracellular tightening against ions, small and large molecules. Cell. Mol. Life Sci. 67(12): 2131-2140 (IF 7.0) [PubMed] [WebPage] [PDF]
Westphal JK, Dörfel MJ, Krug SM, Cording JD, Piontek J, Blasig IE, Tauber R, Fromm M, Huber O (2010) Tricellulin forms homomeric and heteromeric tight junctional complexes. Cell. Mol. Life Sci. 67(12): 2057-2068 (IF 7.0) [PubMed] [WebPage] [Supplement] [PDF]
Zhang J, Piontek J, Wolburg H, Piehl C, Liss M, Otten C, Christ A, Willnow TE, Blasig IE, Abdelilah-Seyfried S (2010) Establishment of a
neuroepithelial barrier by claudin-5a is essential for zebrafish brain ventricular lumen expansion.
Proc. Natl. Acad. Sci.
107(4): 1425-1430
(IF 9.8) [PubMed] [WebPage]
[PDF]
Review
Haseloff RF, Piontek J, Blasig IE (2010) The investigation of cis- and trans-interactions between claudins. Curr. Top. Membr. 65: 97-112, doi.org/10.1016/S1063-5823(10)65005-3
Veshnyakova A, Protze J, Rossa J, Blasig IE, Krause G, Piontek J (2010) On the interaction of clostridium perfringens enterotoxin with claudins. Toxins 2(6): 1336-1356 (IF2012 2.1) [PubMed] [WebPage] [PDF]
2009
Winkler L, Gehring C, Wenzel A, Müller SL, Piehl C, Krause G, Blasig IE*, Piontek J* (*shared last authorship) (2009) Molecular determinants of the interaction between clostridium perfringens enterotoxin fragments and claudin-3. J. Biol. Chem. 284(28): 18863-18872 (IF 5.3) [PubMed] [WebPage] [PDF]
Lohrberg D, Krause E, Schümann M, Piontek J, Winkler L, Blasig IE, Haseloff RF (2009) A strategy for enrichment of claudins based on their affinity to Clostridium perfringens enterotoxin. BMC Mol. Biol. 10(1): 61 (IF 2.8) [PubMed] [WebPage] [PDF] [Supplement]
Walter JK, Castro V, Voss M, Gast K, Rueckert C, Piontek J, Blasig IE (2009) Redox-sensitivity of the dimerization of occludin.
Cell. Mol. Life Sci. 66(22): 3655-3662
(IF 6.1) [PubMed] [WebPage]
[PDF] Review
s
Krause G, Winkler L, Piehl C, Blasig I, Piontek J, Müller SL (2009) Structure and function of extracellular claudin domains. Ann. N.Y. Acad. Sci. 1165: 34-43 (IF 2.7) [PubMed] [WebPage] [PDF]
Walter JK, Rückert C, Voss M, Müller SL, Piontek J, Blasig I (2009) The oligomerization of the coiled-coil domain of occludin is redox-sensitive. Ann. N.Y. Acad. Sci. 1165: 19-27 (IF 2.7) [PubMed] [WebPage] [PDF]
2006 - 2008
Piontek J*, Winkler L* (*shared first authorship), Wolburg H, Müller SL, Zuleger N, Piehl C, Wiesner B, Krause G, Blasig IE (2008) Formation of tight junction: determinants of homophilic interaction between classic claudins. FASEB J. 22(1): 146-158 (IF 7.0) [PubMed] [WebPage] [PDF]
Blasig IE, Winkler L, Lassowski B, Mueller SL, Zuleger N, Krause E, Krause G, Gast K, Kolbe M, Piontek J (2006) On the self-association potential of transmembrane tight junction proteins. Cell. Mol. Life Sci. 63: 505-514 (IF 4.7) [PubMed] [WebPage] [PDF]
Schreibelt G, Kooij G, Reijerkerk A, van Doorn R, Gringhuis SI, van der Pol SMA, Weksler BB, Romero IE, Couraud PQ, Piontek J, Blasig IE, Dijkstra CD, Ronken E, de Vries HE (2007) Reactive oxygen species alter brain endothelial tight junction dynamics via RhoA, PI3 kinase and PKB signaling. FASEB J. 21: 3666-3676 (IF 6.8) [PubMed] [WebPage] [PDF]
Andreeva AY,
Piontek J, Blasig IE, Utepbergenov DI (2006) Assembly of tight junction is regulated by the antagonism of conventional and novel protein kinase C isoforms. Int. J.
Biochem. Cell Biol.
38: 222-233
(IF 4.8)
[PubMed] [WebPage] [PDF] Review
Krause G, Winkler L, Müller SL, Haseloff RF, Piontek J, Blasig IE (2008) Structure and function of claudins. Biochim. Biophys. Acta 1778(3): 631-645 (IF 4.2) [PubMed] [WebPage] [PDF]
1999 - 2003
Piontek J, Brandt R (2003) Differential and regulated binding of cAMP-dependent protein kinase and protein kinase C isoenzymes to gravin in human model neurons: Evidence that gravin provides a dynamic platform for the localization for kinases during neuronal development. J. Biol. Chem. 278(40): 38970-38979 (IF 6.5) [PubMed] [WebPage] [PDF]
Piontek J, Régnier-Vigouroux A, Brandt R (2002) Contact with astroglial membranes induces axonal and dendritic growth of human CNS model neurons and affects the distribution of the growth-associated proteins MAP1B and GAP43. J. Neurosci. Res. 67(4): 471-483 (IF 3.0) [PubMed] [WebPage] [PDF]
Piontek J, Chen CC, Kempf M, Brandt R (1999) Neurotrophins differentially regulate the survival and morphological complexity of human CNS model neurons. J. Neurochem. 73: 139-146 (IF 4.0) [PubMed] [WebPage] [PDF]
Theses
Piontek J (2002) Generation of monoclonal antibodies against proteins of the neuronal membrane cortex and investigation of gravin-signaling complexes in human model neurons. PhD thesis (Dr. rer. nat.), Interdisciplinary Center for Neurosciences (IZN), University of Heidelberg
Piontek J (1997) Analysis of the expression and localization of microtubule-associated proteins in human model neurons. Diploma thesis (Dipl. Biol.), University of Heidelberg
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